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bcip nbt alkaline phosphatase substrate kit  (Vector Laboratories)


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    Vector Laboratories bcip nbt alkaline phosphatase substrate kit
    Bcip Nbt Alkaline Phosphatase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 481 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bcip nbt alkaline phosphatase substrate kit/product/Vector Laboratories
    Average 94 stars, based on 481 article reviews
    bcip nbt alkaline phosphatase substrate kit - by Bioz Stars, 2026-06
    94/100 stars

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    Differentiation features upon DM treatment. (A) Alkaline phosphatase (ALP) activity visualized at day 7 by histological staining. Scale bar: 100 µm. (B) ALP enzymatic assay measured at day 7 and normalized to Crystal violet signal for cell content. (C). Gene expression of SFTP‐C after 7 days in SM or DM analyzed by RT‐qPCR (* p < 0.01, *** p < 0.0001). (D) Immunostaining showing SFTP‐C signal (red) with DAPI (blue) used as nuclear counterstain in A549 treated for 7 and 10 days. Scale bar: 50 µm.

    Journal: Advanced Biology

    Article Title: Differentiation Treatment Applied to Lung Cancer Model Reduces Pathogenic Traits in Vitro

    doi: 10.1002/adbi.202500371

    Figure Lengend Snippet: Differentiation features upon DM treatment. (A) Alkaline phosphatase (ALP) activity visualized at day 7 by histological staining. Scale bar: 100 µm. (B) ALP enzymatic assay measured at day 7 and normalized to Crystal violet signal for cell content. (C). Gene expression of SFTP‐C after 7 days in SM or DM analyzed by RT‐qPCR (* p < 0.01, *** p < 0.0001). (D) Immunostaining showing SFTP‐C signal (red) with DAPI (blue) used as nuclear counterstain in A549 treated for 7 and 10 days. Scale bar: 50 µm.

    Article Snippet: ALP staining was performed with the SK‐5400 detection kit (Vector Laboratories) according to the manufacturer's instructions, and imaged on a Leica DMIL microscope.

    Techniques: Activity Assay, Staining, Enzymatic Assay, Gene Expression, Quantitative RT-PCR, Immunostaining

    Spheroids formation in 3D cultures of A549 in SM or DM. (A) A549 spheroids at day 7 (top) and 14 (bottom). Scale bar: 500 µm. (B) Phalloidin (red) staining of day 7 spheroids in SM or DM medium, with DAPI nuclear counterstain. Scale bar: 500 µm. (C) Spheroid diameter measured over 14 days. (D) RT‐qPCR expression analysis of stem cell markers SOX2, CD44, and NANOG at day 7. (E) ALP staining (dark blue) in spheroids processed by Cytospin. Scale bar: 100 µm. (* p < 0.01, ** p < 0.001, *** p < 0.0001).

    Journal: Advanced Biology

    Article Title: Differentiation Treatment Applied to Lung Cancer Model Reduces Pathogenic Traits in Vitro

    doi: 10.1002/adbi.202500371

    Figure Lengend Snippet: Spheroids formation in 3D cultures of A549 in SM or DM. (A) A549 spheroids at day 7 (top) and 14 (bottom). Scale bar: 500 µm. (B) Phalloidin (red) staining of day 7 spheroids in SM or DM medium, with DAPI nuclear counterstain. Scale bar: 500 µm. (C) Spheroid diameter measured over 14 days. (D) RT‐qPCR expression analysis of stem cell markers SOX2, CD44, and NANOG at day 7. (E) ALP staining (dark blue) in spheroids processed by Cytospin. Scale bar: 100 µm. (* p < 0.01, ** p < 0.001, *** p < 0.0001).

    Article Snippet: ALP staining was performed with the SK‐5400 detection kit (Vector Laboratories) according to the manufacturer's instructions, and imaged on a Leica DMIL microscope.

    Techniques: Staining, Quantitative RT-PCR, Expressing